Mitalipov Sh M, Fedorov L M, Strel'chenko N S
Ontogenez. 1993 Sep-Oct;24(5):5-10.
Mouse chimeras were produced using injections of ICM cells into blastocysts. Chimerism of resulting animals was determined by their coat color and spectrum of glucosephosphate isomerase isoenzymes. The use of modifications of the injection method for solving different genetic and embryological problems is discussed.
通过将内细胞团(ICM)细胞注射到囊胚中来制备小鼠嵌合体。通过其毛色和葡萄糖磷酸异构酶同工酶谱来确定所得动物的嵌合情况。本文讨论了使用注射方法的改进来解决不同的遗传和胚胎学问题。