Nitta H, Ishikawa I
Department of Periodontology, Faculty of Dentistry, Tokyo Medical and Dental University, Japan.
Oral Microbiol Immunol. 1993 Jun;8(3):141-5. doi: 10.1111/j.1399-302x.1993.tb00656.x.
Genetic regulation of the immune response may be involved in the onset and progression of an early-onset type of periodontitis. We analyzed the genetic control of the primary antibody response to Actinobacillus actinomycetemcomitans in inbred strains of mice using an immunoblot technique. Mice of 5 independent inbred strains, 6 H-2 congenic strains and 4 B10 intra-H-2 recombinant strains were immunized with sonicated extracts of A. actinomycetemcomitans. On the seventh day their sera were examined for reactivity to the antigenic components of this organism. Western blot analysis clearly distinguished 2 different groups of antigens, one consisting of common antigens (molecular weights, 28, 34, 36 and 40 kDa) that reacted with sera from all strains and one consisting of specific antigens (molecular weights 31, 65 and 69 kDa) that reacted only with sera from distinct strains. Blot analysis of sera from H-2 congenic strains demonstrated that the reactivity to the second group of antigens was regulated by the H-2 complex. In B10 intra-H-2 recombinant strains, only the I-Ab allotype strains produced immunoglobulin G antibody that reacted to the 65 kDa antigen. This evidence indicates that the primary immune response to the A. actinomycetemcomitans antigen with a molecular weight of 65 kDa is controlled by genes in the I-A subregion of the H-2 complex. This 65 kDa antigen was also highly reactive with some human sera from early-onset periodontitis patients. Further analysis of this antigen is required.
免疫反应的基因调控可能与早发性牙周炎的发病和进展有关。我们使用免疫印迹技术分析了近交系小鼠对伴放线放线杆菌的初次抗体反应的基因控制。用伴放线放线杆菌的超声提取物免疫5个独立近交系、6个H-2同源近交系和4个B10 H-2内部重组系的小鼠。在第7天，检测它们的血清对该菌抗原成分的反应性。蛋白质印迹分析清楚地区分出两组不同的抗原，一组是由与所有品系血清都反应的共同抗原（分子量分别为28、34、36和40 kDa）组成，另一组是仅与不同品系血清反应的特异性抗原（分子量为31、65和69 kDa）组成。对H-2同源近交系血清的印迹分析表明，对第二组抗原的反应性受H-2复合体调控。在B10 H-2内部重组系中，只有I-Ab同种异型品系产生了与65 kDa抗原反应的免疫球蛋白G抗体。这一证据表明，对分子量为65 kDa的伴放线放线杆菌抗原的初次免疫反应受H-2复合体I-A亚区的基因控制。这种65 kDa抗原也与一些早发性牙周炎患者的人血清有高度反应性。需要对该抗原进行进一步分析。
