Piggins H D, Moody T W, Merali Z
School of Psychology, University of Ottawa, Ontario, Canada.
Peptides. 1993 Jul-Aug;14(4):845-8. doi: 10.1016/0196-9781(93)90123-x.
Long-term consequences of neonatal blockade of bombesin (BN) receptors were examined in the present study. Rat pups were injected twice daily with [D-Phe6, phi Leu13-Cpa14]BN(6-14), a BN receptor antagonist, at either high (10 mg/kg; HD group) or low (5 mg/kg; LD group) doses from postnatal day 1 through 8. Their behavioral responses to a variety of conditions were compared to those of rats neonatally injected with saline (SAL group) or animals handled but not injected during infancy (UNT group). Adult HD rats entered and spent more time on the open arms of the elevated plus maze than LD, SAL, or UNT animals. Under the conditions of a water deprivation schedule, neither central nor peripheral injections of BN differentiated the neonatally pretreated groups as determined by measures of grooming, feeding, and drinking behaviors. These results indicate that at the dosage regimen employed, neonatal injections of [D-Phe6, phi Leu13-Cpa14]BN(6-14) had little effect on adult sensitivity to BN, but that such treatments could alter activity on the elevated plus maze through as yet unknown mechanisms.
本研究检测了新生大鼠中铃蟾肽（BN）受体阻断的长期后果。从出生后第1天至第8天，给幼鼠每日两次注射[D-苯丙氨酸6，苯丙氨酸亮氨酸13-半胱氨酸丙氨酸14]BN（6-14），一种BN受体拮抗剂，剂量分别为高剂量（10 mg/kg；HD组）或低剂量（5 mg/kg；LD组）。将它们对各种条件的行为反应与新生期注射生理盐水的大鼠（SAL组）或幼年期接受抓握但未注射的动物（UNT组）进行比较。成年HD大鼠在高架十字迷宫开放臂上进入和停留的时间比LD组、SAL组或UNT组动物更长。在禁水方案条件下，通过梳理、进食和饮水行为测量发现，无论是中枢还是外周注射BN都无法区分新生期预处理组。这些结果表明，在所采用的剂量方案下，新生期注射[D-苯丙氨酸6，苯丙氨酸亮氨酸13-半胱氨酸丙氨酸14]BN（6-14）对成年大鼠对BN的敏感性影响不大，但这种处理可能通过尚不清楚的机制改变高架十字迷宫中的活动。
