Rygg M, Husby G, Marhaug G
Institute of Clinical Medicine, University of Tromsø, Norway.
Scand J Immunol. 1993 Nov;38(5):417-22. doi: 10.1111/j.1365-3083.1993.tb02582.x.
Serum amyloid A (SAA) is an acute-phase plasma protein which increases up to 1000-fold after an acute-phase stimulus. Several SAA genes and corresponding protein isotypes exist in individual species. Liver is the main source of production, but extra-hepatic SAA expression has been described. In this study inflammation was induced in rabbits with lipopolysaccharide, turpentine, or casein. Transcription of SAA mRNA was studied using Northern blot analysis with probes specific for three different rabbit SAA isotypes and analysed by scanning densitometry. In the stimulated liver slight variation in SAA mRNA transcription level was seen after stimulation with different inflammatory agents. After lipopolysaccharide-stimulation SAA gene expression was also seen in most of the extra-hepatic organs. After turpentine stimulation SAA mRNA was seen in the liver, the ovary, and the small intestines, and after casein stimulation only in the liver and the ovary. SAA1 and SAA2 were induced exclusively in the liver, while SAA3 was induced mainly in the extra-hepatic organs. This indicates that the SAA genes probably are independently regulated both in relation to stimulus, gene- and tissue-specificity.
血清淀粉样蛋白A（SAA）是一种急性期血浆蛋白，在急性期刺激后可增加至1000倍。单个物种中存在几种SAA基因及相应的蛋白同种型。肝脏是主要的产生来源，但已报道存在肝外SAA表达。在本研究中，用脂多糖、松节油或酪蛋白诱导兔发生炎症。使用针对三种不同兔SAA同种型的探针，通过Northern印迹分析研究SAA mRNA的转录，并通过扫描密度测定法进行分析。在受刺激的肝脏中，用不同的炎症因子刺激后，SAA mRNA转录水平有轻微变化。脂多糖刺激后，大多数肝外器官也出现SAA基因表达。松节油刺激后，在肝脏、卵巢和小肠中可见SAA mRNA，酪蛋白刺激后仅在肝脏和卵巢中可见。SAA1和SAA2仅在肝脏中被诱导，而SAA3主要在肝外器官中被诱导。这表明SAA基因可能在刺激、基因和组织特异性方面受到独立调节。
