Seymour J L, Schmidt S P, Allen J R
Proc Soc Exp Biol Med. 1976 Sep;152(4):621-5. doi: 10.3181/00379727-152-39454.
Incubation of tritiated 2,5,2',5'-tetrachlorobiphenyl with normal monkey liver microsomes in a NADPH-generating system results in the formation of active metabolite(s) of the [3H]2,5,2',5'-tetrachlorobiphenyl capable of covalently binding to Rna and protein isolated from the incubation mixture. The metabolite is not formed when the control microsomes are held at 100 degrees for 10 min prior to incubation. The addition of microsomal supernate to the solution causes an increase in the binding of the active metabolite to macromolecules while the addition of glutathione to the incubation medium significantly inhibits this increase.
在一个生成NADPH的系统中，将氚标记的2,5,2',5'-四氯联苯与正常猴肝微粒体一起温育，会导致[3H]2,5,2',5'-四氯联苯的活性代谢物形成，该代谢物能够与从温育混合物中分离出的RNA和蛋白质共价结合。在温育前将对照微粒体在100摄氏度下保持10分钟，则不会形成该代谢物。向溶液中添加微粒体上清液会导致活性代谢物与大分子的结合增加，而向温育培养基中添加谷胱甘肽则会显著抑制这种增加。
