Yamamoto J, Ishii I, Okada Y, Yamashita T, Ridler C D, Gorog P, Kovacs I B
Laboratory of Physiology, Faculty of Nutrition, Kobe-Gakuin University, Japan.
Thromb Res. 1993 Aug 15;71(4):281-7. doi: 10.1016/0049-3848(93)90197-v.
Contribution of leucocytes to formation and lysis of arterial (platelet) thrombi was investigated. Secretory products of polymorphonuclear leucocytes (PMNs) during phagocytosis and cell lysates were prepared from eight volunteers. Platelet-rich thrombi were formed in flowing native human blood either by shear-stress or by collagen fibre, by haemostatometry. Tested in eight volunteers, PMN products significantly enhanced both thrombotic reactions and induced lysis of these thrombi. A specific inhibitor of leucocyte proteases (eglin c) inhibited platelet reaction to shear-stress and collagen and spontaneous thrombolysis. Our findings provide further evidence for the prothrombotic and potent thrombolytic effect of leucocytes associated with an arterial thrombus.
