[Morphogenesis of Bacillus sphaericus Ts-1 and formation of toxic proteins to mosquito larva].

The close relationships between morphogenesis of Bacillus sphaericus Ts-1 and formation of toxic proteins were studied systemically and comparatively by Enzyme-Linked Immunosorbent Assay, FITC-IgG Location and SDS-PAGE etc. This study showed that the toxic proteins of Ts-1 were produced in 8h culture at 30 degrees C. The toxicity increased steadily along with the sporification and then declined as the free spores increased greatly without collection immediately. It is suggested that substances homologous with crystal toxic proteins were situated on the cell walls.