Zhang B, Ren G, Wang J
Department of Biology, Nankai University, Tianjin.
Wei Sheng Wu Xue Bao. 1993 Jun;33(3):210-3.
The close relationships between morphogenesis of Bacillus sphaericus Ts-1 and formation of toxic proteins were studied systemically and comparatively by Enzyme-Linked Immunosorbent Assay, FITC-IgG Location and SDS-PAGE etc. This study showed that the toxic proteins of Ts-1 were produced in 8h culture at 30 degrees C. The toxicity increased steadily along with the sporification and then declined as the free spores increased greatly without collection immediately. It is suggested that substances homologous with crystal toxic proteins were situated on the cell walls.
通过酶联免疫吸附测定、异硫氰酸荧光素标记的免疫球蛋白定位和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳等方法，对球形芽孢杆菌Ts-1的形态发生与毒性蛋白形成之间的密切关系进行了系统和比较研究。该研究表明，Ts-1的毒性蛋白在30℃培养8小时时产生。随着芽孢形成，毒性稳步增加，然后随着游离芽孢大量增加且未立即收集而下降。结果表明，与晶体毒性蛋白同源的物质位于细胞壁上。
