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茶叶中黄酮碳苷的测定

Determination of flavone C-glycosides in tea.

作者信息

Engelhardt U H, Finger A, Kuhr S

机构信息

Institut für Lebensmittelchemie, Braunschweig, Germany.

出版信息

Z Lebensm Unters Forsch. 1993 Sep;197(3):239-44. doi: 10.1007/BF01185278.

Abstract

An HPLC method for the determination of flavone C-glycosides (FCG) from black tea has been developed. Sample clean-up was accomplished by means of polyamide column chromatography, followed by enzyme hydrolysis of interfering compounds such was flavonol glycosides and a second polyamide column chromatographic step. Using HPLC with gradient elution and photodiode array detection eight FCG were separated. Seven FCG were isolated by means of preparative HPLC. Identification was carried out using co-chromatography, FAB(Fast Atom Bombardment)-mass spectrometry and various nuclear magnetic resonance techniques. Apigenin 6-C-glucosyl-8-arabinoside (schaftoside) and apigenin 6-C-arabinosyl-8-C-glucoside (isoschaftoside) as well as luteolin 8-C-glucoside (orientin) and luteolin 6-C-glucoside (isoorientin) have been detected in tea for the first time. Three of the other compounds have been identified as apigenin 8-C-glucoside (vitexin), apigenin 6-C-glucoside (isovitexin) and apigenin 6,8-di-C-glucoside (vicenin-2). Their occurrence in tea has been previously reported. From its UV spectrum another compound was concluded to be an apigenin glycoside. The FCG were quantified in a variety of teas of different origins (16 black, two green and one oolong). The total amounts of the FCG were 0.48-2.69 g/kg dry weight. The FCG pattern of teas of different origins were similar to each other and no origin-dependent characteristics have yet been observed. Small amounts of FCG (1.2-2.2 mg/kg) were detected in hydrolysates of high relative molecular mass fractions (Mr > 5000) of a black tea liquor.

摘要

已开发出一种用于测定红茶中黄酮碳苷(FCG)的高效液相色谱法。通过聚酰胺柱色谱法进行样品净化,随后对黄酮醇苷等干扰化合物进行酶水解,并进行第二次聚酰胺柱色谱步骤。使用具有梯度洗脱和光电二极管阵列检测的高效液相色谱法分离出8种FCG。通过制备型高效液相色谱法分离出7种FCG。使用共色谱法、快原子轰击(FAB)质谱法和各种核磁共振技术进行鉴定。首次在茶叶中检测到芹菜素6-C-葡萄糖基-8-阿拉伯糖苷(schaftoside)和芹菜素6-C-阿拉伯糖基-8-C-葡萄糖苷(异schaftoside)以及木犀草素8-C-葡萄糖苷(orientin)和木犀草素6-C-葡萄糖苷(异orientin)。其他三种化合物已鉴定为芹菜素8-C-葡萄糖苷(牡荆素)、芹菜素6-C-葡萄糖苷(异牡荆素)和芹菜素6,8-二-C-葡萄糖苷(vicenin-2)。它们在茶叶中的存在此前已有报道。从其紫外光谱得出另一种化合物为芹菜素糖苷。对多种不同产地的茶叶(16种红茶、2种绿茶和1种乌龙茶)中的FCG进行了定量。FCG的总量为0.48 - 2.69 g/kg干重。不同产地茶叶的FCG模式彼此相似,尚未观察到产地依赖性特征。在一种红茶茶汤的高相对分子质量级分(Mr > 5000)的水解产物中检测到少量FCG(1.2 - 2.2 mg/kg)。

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