Bocchini V, Beccari T, Arcuri C, Bruyere L, Fages C, Tardy M
Department of Experimental Medicine, University of Perugia, Italy.
Int J Dev Neurosci. 1993 Aug;11(4):485-92. doi: 10.1016/0736-5748(93)90022-6.
The expression of two astroglial differentiation markers, vimentin and glial fibrillary acidic protein, was investigated in a previously established human glioma cell line of clonal origin (GL15). Vimentin immunolabelling was homogeneously expressed in all cells. Glial fibrillary acidic protein and its encoding message, investigated by immunocytochemistry and in situ hybridization, showed a mosaic-like expression. Only 30% of the cell population expressed glial fibrillary acidic protein and its mRNA. Western and Northern blots performed for both markers confirmed the presence of both proteins and messages, and their level was correlated with the observed antigenic and molecular probe labelling. The overall antigenic pattern suggests that GL-15 cells do not belong to the O-2A progenitor cell lineage and may arise from a clonal expansion of astrocyte precursors.
在先前建立的克隆起源的人胶质瘤细胞系（GL15）中，研究了两种星形胶质细胞分化标志物波形蛋白和胶质纤维酸性蛋白的表达。波形蛋白免疫标记在所有细胞中均呈均匀表达。通过免疫细胞化学和原位杂交研究的胶质纤维酸性蛋白及其编码信息呈镶嵌样表达。仅30%的细胞群体表达胶质纤维酸性蛋白及其mRNA。针对这两种标志物进行的蛋白质免疫印迹和Northern印迹证实了这两种蛋白质及其信息的存在，并且它们的水平与观察到的抗原和分子探针标记相关。整体抗原模式表明，GL - 15细胞不属于少突胶质前体细胞系，可能源自星形胶质细胞前体的克隆性扩增。
