Hafidi A, Després G, Romand R
Laboratoire de Neurobiologie et Physiologie du développement, Université Blaise Pascal, Aubière, France.
Int J Dev Neurosci. 1993 Aug;11(4):507-12. doi: 10.1016/0736-5748(93)90024-8.
In this study, we analysed the distribution of the intermediate filament peripherin in the developing cochlea of the rat. At gestational day 16, weak immunolabeling was observed in neuronal somas throughout the spiral ganglion. At gestational day 20, the peripherin labeling increased in intensity throughout the spiral ganglion. At gestational day 20, the peripherin labeling increased in intensity throughout the cochlea but became especially strong in some ganglion neurons of the basal turn. Homogeneous immunolabeling was observed throughout the spiral ganglion of the apical turn. Double immunofluorescence labeling of the prenatal cochlea with peripherin and neurofilament (NF) antibodies revealed colocalization on the same structures. By postnatal day 3, the peripherin labeling intensity had decreased in the majority of spiral ganglion neurons, but remained strong in some cells of the basal turn. Only a few neurons continued to be immunolabeled into adulthood that correspond to Type II spiral ganglion neurons expressing both NF protein and peripherin, two classes of intermediate filament proteins. In the organ of Corti, the first immunolabeling was observed on gestational day 20 as peripheral fibers reaching the receptor cells. Positive fibers were observed below both inner (IHCs) and outer (OHCs) hair cells. At birth and at postnatal day 3, peripherin immunolabeling was still observed below both IHCs and OHCs. By postnatal day 4, peripherin labeling became more dominant in fibers below OHCs, but some immunoreactivity was still present below IHCs. No immunoreactivity was present in the intraganglionic spiral bundle (IGSB) fibers containing the olivary complex efferent fibers before birth. A few days after birth some fibers of the IGSB started to be immunoreactive.
在本研究中，我们分析了中间丝外周蛋白在大鼠发育中的耳蜗中的分布情况。在妊娠第16天，在整个螺旋神经节的神经元胞体中观察到微弱的免疫标记。在妊娠第20天，整个螺旋神经节中外周蛋白标记的强度增加。在妊娠第20天，整个耳蜗中外周蛋白标记的强度增加，但在基底转的一些神经节神经元中变得特别强。在顶转的整个螺旋神经节中观察到均匀的免疫标记。用外周蛋白和神经丝（NF）抗体对产前耳蜗进行双重免疫荧光标记，结果显示在相同结构上存在共定位。到出生后第3天，大多数螺旋神经节神经元中外周蛋白标记强度降低，但在基底转的一些细胞中仍很强。只有少数神经元在成年后仍被免疫标记，这些神经元对应于同时表达NF蛋白和外周蛋白这两类中间丝蛋白的II型螺旋神经节神经元。在柯蒂氏器中，在妊娠第20天观察到首次免疫标记，此时外周纤维到达受体细胞。在内毛细胞（IHC）和外毛细胞（OHC）下方均观察到阳性纤维。在出生时和出生后第3天，在IHC和OHC下方仍可观察到外周蛋白免疫标记。到出生后第4天，外周蛋白标记在OHC下方的纤维中变得更加明显，但在IHC下方仍存在一些免疫反应性。在出生前，含有橄榄复合体传出纤维的螺旋神经节内束（IGSB）纤维中不存在免疫反应性。出生几天后，IGSB的一些纤维开始具有免疫反应性。
