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[转氨酶的组织化学证据。V. 大鼠器官中转氨酶的定量组织化学研究(作者译)]

[Histochemical evidence of the aminotransferases. V. Quantitative histochemical investigations of the aminotransferases in organs of the rat (author's transl)].

作者信息

Lolova I, Dikow A, Mladjova A

出版信息

Acta Histochem. 1976;55(2):216-24.

PMID:823766
Abstract

The quantitative histochemical method was employed the activity of some aminotransferases in the rat organs. Determination was based on extraction of the enzymatic reaction product formazan with alkaline dimethylsulfoxid. Evaluation of the enzymatic activity was given by the formazan quantity related to 1 mug tissue protein. The following transaminases have been studied: aspartate-, alanine-, tyrosine-, phenylalanine-, tryptophane-, glycine-, and serine aminotransferases, as the leucine aminotransferase with substrate leucine, isoleucine and valine. Difference was observed as with respect to one and the same aminotransferase in the examined organs, as well, with respect to the different aminotransferases in one and the same organ.

摘要

采用定量组织化学方法研究大鼠器官中某些转氨酶的活性。测定基于用碱性二甲基亚砜提取酶促反应产物甲臜。酶活性通过与1微克组织蛋白相关的甲臜量来评估。研究了以下转氨酶:天冬氨酸转氨酶、丙氨酸转氨酶、酪氨酸转氨酶、苯丙氨酸转氨酶、色氨酸转氨酶、甘氨酸转氨酶和丝氨酸转氨酶,以及以亮氨酸、异亮氨酸和缬氨酸为底物的亮氨酸转氨酶。在所检查的器官中,对于同一种转氨酶以及在同一个器官中的不同转氨酶,均观察到了差异。

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