Jaisser F, Coutry N, Farman N, Binder H J, Rossier B C
Institut de Pharmacologie et de Toxicologie, Université de Lausanne, Switzerland.
Am J Physiol. 1993 Oct;265(4 Pt 1):C1080-9. doi: 10.1152/ajpcell.1993.265.4.C1080.
Recently, a putative distal colon H(+)-K(+)-ATPase alpha-subunit has been identified and characterized (M. S. Crowson and G. E. Shull. J. Biol. Chem. 267:13740-13748, 1992). In the present study, we report the tissue and cell expression of this putative H(+)-K(+)-ATPase. The results indicate that, first, in the gut, the putative H(+)-K(+)-ATPase alpha-subunit is restricted to the distal part of the colon and is predominantly expressed in surface epithelial cells, in marked contrast to the alpha 1-subunit of Na(+)-K(+)-ATPase that is also expressed in glands. These data suggest that the H(+)-K(+)-ATPase alpha-subunit is a potential marker for terminal differentiation of distal colon. Second, in the uterus, the putative H(+)-K(+)-ATPase is restricted to the region of the myometrium between the inner and midmuscular zone that is very rich in vascular supply and nerve cells. This striking expression suggests that the H(+)-K(+)-ATPase may not be involved in the control of pH and potassium concentration of the uterine fluid but rather in distinct functions of vascular and/or nerve cells. Third, with the use of three independent and different approaches (Northern blot analysis, ribonuclease protection assay, and in situ hybridization), we were unable to detect any significant amount of H(+)-K(+)-ATPase transcripts in kidney tissue. Our data suggest that the putative distal colon H(+)-K(+)-ATPase is probably distinct from the kidney isoform. Finally, we report the sequence of a set of degenerate oligonucleotides that are useful to clone alpha-subunits of the Na(+)-K(+)-/H(+)-K(+)-ATPase gene family in different tissues and different species.
最近，一种假定的远端结肠H(+)-K(+)-ATP酶α亚基已被鉴定和表征（M.S.克劳森和G.E.舒尔。《生物化学杂志》267:13740-13748，1992）。在本研究中，我们报告了这种假定的H(+)-K(+)-ATP酶的组织和细胞表达情况。结果表明，首先，在肠道中，假定的H(+)-K(+)-ATP酶α亚基局限于结肠远端部分，主要表达于表面上皮细胞，这与也在腺体中表达的Na(+)-K(+)-ATP酶α1亚基形成显著对比。这些数据表明，H(+)-K(+)-ATP酶α亚基是远端结肠终末分化的潜在标志物。其次，在子宫中，假定的H(+)-K(+)-ATP酶局限于子宫肌层内肌层和中肌层之间血管供应和神经细胞非常丰富的区域。这种显著的表达表明，H(+)-K(+)-ATP酶可能不参与子宫液pH值和钾浓度的调节，而是参与血管和/或神经细胞的独特功能。第三，通过使用三种独立且不同的方法（Northern印迹分析、核糖核酸酶保护分析和原位杂交），我们未能在肾脏组织中检测到任何大量的H(+)-K(+)-ATP酶转录本。我们的数据表明，假定的远端结肠H(+)-K(+)-ATP酶可能与肾脏同工型不同。最后，我们报告了一组简并寡核苷酸的序列，这些序列有助于克隆不同组织和不同物种中Na(+)-K(+)/H(+)-K(+)-ATP酶基因家族的α亚基。
