Liu H, Miwa M, Beauvoit B, Wang N G, Chance B
Johnson Research Foundation, Department of Biochemistry and Biophysics, School of Medicine, University of Pennsylvania, Philadelphia 19104-6089.
Anal Biochem. 1993 Sep;213(2):378-85. doi: 10.1006/abio.1993.1435.
With time-resolved spectroscopy, we develop an experimental approach by sample substitution to measure the absorption (mu a) and reduced scattering (mu's) coefficients of small-volume biological samples. To investigate the method, small-volume control samples are substituted into a large-size host medium during increases in the absorber (or scatterer) concentration of the host. By characterizing the deviation of the spectra taken with and without the sample, we determine the matching points where the sample and surrounding medium are optically identical. We show that this method can result in correct values of the mu a and mu's for the sample within 6% error if the matching conditions for both the mu a and mu's are fully realized. The results also indicate that this method can give approximate values of the mu a and mu's in a reasonable range if either the mu a or the mu's matching between the two media is realized. This method has been applied to the studies of absorption properties of a human finger and of scattering properties of yeast.
利用时间分辨光谱技术，我们开发了一种通过样品替换来测量小体积生物样品吸收系数（μa）和约化散射系数（μ's）的实验方法。为了研究该方法，在主体吸收体（或散射体）浓度增加的过程中，将小体积对照样品替换到大型主体介质中。通过表征有无样品时所采集光谱的偏差，我们确定样品与周围介质光学性质相同的匹配点。我们表明，如果μa和μ's的匹配条件都得到充分满足，该方法能够得出样品的μa和μ's的正确值，误差在6%以内。结果还表明，如果两种介质之间实现了μa或μ's的匹配，该方法能够在合理范围内给出μa和μ's的近似值。该方法已应用于人体手指吸收特性和酵母散射特性的研究。
