Ellis J A, Coen M L, MacLachlan N J, Wilson W C, Williams E S, Leudke A J
Department of Veterinary Sciences, University of Wyoming, Laramie 82070.
Am J Vet Res. 1993 Sep;54(9):1452-6.
Replication of bluetongue virus (BTV) in leukocytes from the blood of sheep, cattle, elk, and mule deer inoculated with BTV serotype 10 or 17 was assessed by immunocytochemical staining and dot blot northern hybridization to determine if differences in the prevalence of infection in this blood fraction might account for the differences in clinical disease among these species. Viremia was confirmed by virus isolation in all inoculated animals. Analysis of leukocytes with monoclonal antibodies specific for BTV proteins revealed low numbers of infected leukocytes in only 2 sheep 8 days after inoculation with BTV serotype 10. Most of the cells expressing BTV were identified morphologically as monocytes; approximately 10% of infected cells were lymphocytes. Bluetongue virus was not detected by use of dot-blot hybridization on samples of blood. Our results suggest that differential infection of leukocytes does not account for the pronounced differences in clinical signs and pathologic changes among ruminants.
通过免疫细胞化学染色和斑点印迹Northern杂交，评估了接种蓝舌病病毒（BTV）血清型10或17的绵羊、牛、麋鹿和骡鹿血液中白细胞内BTV的复制情况，以确定该血液组分中感染率的差异是否可以解释这些物种临床疾病的差异。通过病毒分离在所有接种动物中均证实了病毒血症。用针对BTV蛋白的单克隆抗体分析白细胞发现，接种BTV血清型10后8天，只有2只绵羊中有少量受感染的白细胞。大多数表达BTV的细胞在形态上被鉴定为单核细胞；约10%的受感染细胞为淋巴细胞。在血液样本上使用斑点印迹杂交未检测到蓝舌病病毒。我们的结果表明，白细胞的差异感染并不能解释反刍动物临床症状和病理变化的显著差异。
