Peloso J G, Stick J A, Soutas-Little R W, Caron J C, DeCamp C E, Leach D H
Department of Large Animal Clinical Sciences, Michigan State University, East Lansing 48824-1314.
Am J Vet Res. 1993 Sep;54(9):1535-43.
Motion of 6 clinically sound horses trotting at a speed of 4 m/s on a treadmill was captured by video cameras before and 9, 16, and 23 days after amphotericin-induced lameness to determine the quantitative variables of three-dimensional computer-assisted image analysis that objectively describe carpal lameness. Amphotericin-B was used to induce lameness, and phenylbutazone (2.2 mg/kg of body weight, PO, once) and butorphanol tartrate (0.1 mg/kg IM, q 6 h, to effect) were used to control discomfort. Four 60-Hz cameras were symmetrically placed around the treadmill to capture 6 seconds of images from retro-reflective spheres taped to the trotting horses. Images were transferred to a video-based digitizer and a computer work station, where 4 files of two-dimensional data were reduced to 1 file of three-dimensional data. The effect of lameness on motion analyzed was assessed by use of two-way ANOVA. Differences between means were assessed, using the Student-Newman-Keul's test (P < or = 0.05). Head and withers excursions, (dorsal vertical displacement of head and withers targets, respectively) during the sound forelimb support phase were increased significantly during all lameness measurement periods. Head excursion, but not withers excursion, during the lame forelimb support phase, was decreased significantly during all lameness measurement periods. Computer determinations of stride length swing phase, stance phase, forelimb abduction, and carpal and fetlock ranges of motion did not consistently characterize the lameness. It was concluded that three-dimensional computer-assisted image analysis could be used for objective lameness evaluation in horses and that head and withers excursions were the most consistent variables for assessing equine carpal lameness.
在跑步机上，用摄像机捕捉6匹临床健康的马以4米/秒的速度小跑时的运动情况，分别在两性霉素诱导跛行前以及跛行后9天、16天和23天进行，以确定三维计算机辅助图像分析的定量变量，从而客观描述腕关节跛行。使用两性霉素B诱导跛行，用苯基丁氮酮（2.2毫克/千克体重，口服，一次）和酒石酸布托啡诺（0.1毫克/千克，肌肉注射，每6小时一次，至起效）控制不适。四个60赫兹的摄像机对称放置在跑步机周围，以捕捉贴在小跑马匹上的反光球6秒钟的图像。图像被传输到基于视频的数字化仪和计算机工作站，在那里，4个二维数据文件被缩减为1个三维数据文件。使用双向方差分析评估跛行对所分析运动的影响。使用Student-Newman-Keul检验评估均值之间的差异（P≤0.05）。在所有跛行测量期间，健全前肢支撑阶段的头部和鬐甲偏移（分别为头部和鬐甲目标的背侧垂直位移）显著增加。在所有跛行测量期间，患侧前肢支撑阶段的头部偏移显著减少，但鬐甲偏移未减少。计算机对步幅摆动期、站立期、前肢外展以及腕关节和跗关节活动范围的测定并不能始终如一地表征跛行情况。得出的结论是，三维计算机辅助图像分析可用于马匹的客观跛行评估，并且头部和鬐甲偏移是评估马腕关节跛行最一致的变量。
