Phillips L G, Abdullah K M, Geldner P D, Dobbins S, Ko F, Linares H A, Broemeling L D, Robson M C
Division of Plastic Surgery, University of Texas Medical Branch, Galveston 77550.
Ann Plast Surg. 1993 Oct;31(4):331-4. doi: 10.1097/00000637-199310000-00009.
This study examines the effect of application of basic fibroblast growth factor (bFGF) on the wound healing impairment seen in streptozotocin-induced diabetic (SD) rats compared with control nondiabetic (NL) animals. Under general anesthesia, a 6-cm dorsal incision was made through the skin and panniculus carnosus. Both the NL and SD wounds were injected with 0.1 ml of one of the following three solutions: saline, vehicle, and 10 micrograms of bFGF. The wounds were closed with interrupted sutures of 4-0 nylon. The animals were returned to their cages and sacrificed at 7, 10, 14, or 21 days later. Breaking strength of the wound was analyzed by using an Instron Tensiometer 4201 to assay for relative collagen maturation. Data among groups were compared using an analysis of variance. At 7 and 10 days, all NL wounds were stronger than all SD wounds. By 14 days there was no statistically significant difference in breaking strength between the bFGF-SD wounds and all subgroups of NL wounds. The bFGF-SD subgroup was statistically significantly stronger than the saline-SD (p < 0.02) and vehicle-SD (p < 0.01) wounds. At 21 days the bFGF-SD wounds were statistically significantly stronger than the vehicle-SD wounds to a confidence level of p < 0.001. These findings indicate that application of bFGF may reverse the impairment seen in diabetic wound healing.
本研究检测了与对照非糖尿病（NL）动物相比，应用碱性成纤维细胞生长因子（bFGF）对链脲佐菌素诱导的糖尿病（SD）大鼠伤口愈合受损情况的影响。在全身麻醉下，通过皮肤和腹直肌在背部做一个6厘米的切口。NL和SD伤口均注射0.1毫升以下三种溶液之一：生理盐水、赋形剂和10微克bFGF。伤口用4-0尼龙间断缝合关闭。将动物放回笼中，并在7、10、14或21天后处死。使用英斯特朗4201型拉力试验机分析伤口的抗张强度，以检测相对胶原成熟度。使用方差分析比较各组数据。在第7天和第10天，所有NL伤口均比所有SD伤口更强。到第14天，bFGF-SD伤口与NL伤口的所有亚组之间在抗张强度上无统计学显著差异。bFGF-SD亚组在统计学上显著强于生理盐水-SD组（p<0.02）和赋形剂-SD组（p<0.01）伤口。在第21天，bFGF-SD伤口在统计学上显著强于赋形剂-SD伤口，置信水平为p<0.001。这些发现表明，应用bFGF可能会逆转糖尿病伤口愈合中出现的损伤。
