Roberts G P, Parker J M
Biochim Biophys Acta. 1976 Sep 28;446(1):69-76. doi: 10.1016/0005-2795(76)90098-2.
Serum, allantoic fluid and uterine washings obtained from cows at various stages of pregnancy were fractionated by gel filtration and the fractions compared by electrophoretic and immunological methods. Several proteins were detected in uterine washings which were not found in serum. Some of these proteins were sensitive to the experimental conditions used for concentrating the uterine washings, being irreversibly adsorbed onto the glass containers on freeze drying. The number and imtensity of the "uterine-specific" proteins varied with the stage of pregnancy, changes in the electrophoretic patterns being detected just prior to rapid elongation of the embryo and at a stage when luteolysis would have occurred in the unmated cow. Many of the "uterine-specific" proteins were also detected in allantoic fluid.
从处于不同妊娠阶段的奶牛身上获取血清、尿囊液和子宫冲洗液，通过凝胶过滤进行分级分离，并采用电泳和免疫学方法对各组分进行比较。在子宫冲洗液中检测到了几种血清中未发现的蛋白质。其中一些蛋白质对用于浓缩子宫冲洗液的实验条件敏感，冻干时会不可逆地吸附在玻璃容器上。“子宫特异性”蛋白质的数量和强度随妊娠阶段而变化，在胚胎快速伸长之前以及未交配奶牛会发生黄体溶解的阶段，检测到电泳图谱发生了变化。在尿囊液中也检测到了许多“子宫特异性”蛋白质。
