对与鸡A1(1)胶原蛋白基因cDNA同源的产朊假丝酵母基因组DNA的分析。
Analysis of Candida utilis genomic DNA, homologous to cDNA of chicken A1(1) collagen gene.
作者信息
Nurmiskaya M V, Kalebina T S, Nurminsky D I, Kulaev I S
机构信息
Department of Molecular Biology, Biological Faculty, Moscow State University, Russia.
出版信息
Arch Microbiol. 1993;160(4):329-31. doi: 10.1007/BF00292086.
PMID:8239884
Abstract
Genomic fragments, homologous to chicken A1(1) collagen cDNA encoding triple-helical domain, were revealed by Southern analysis in various fungi. Such a genomic fragment from Candida utilis was cloned and sequenced. Analysis of the obtained DNA sequence revealed the 119 bp segment, which has possibly originated from the 54 bp module common for the fibrillar collagen genes of higher eukaryotes.
摘要
通过Southern分析在多种真菌中发现了与编码三螺旋结构域的鸡A1(1)胶原cDNA同源的基因组片段。克隆并测序了来自产朊假丝酵母的这样一个基因组片段。对所得DNA序列的分析揭示了一个119 bp的片段，它可能起源于高等真核生物纤维状胶原基因共有的54 bp模块。
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