Weinberg D S, Weidner N
Department of Pathology, Brigham and Women's Hospital, Boston, Mass. 02115.
Arch Pathol Lab Med. 1993 Nov;117(11):1132-7.
It has been postulated that prostatic intraepithelial neoplasia (PIN) is a precursor lesion to invasive carcinoma in the prostate, yet there has been little direct evidence for this relationship. Therefore, we studied the DNA content of the epithelial cells in PIN lesions with accompanying invasive carcinoma in seven cases. DNA content of nuclei in Feulgen-stained tissue sections was performed using the CAS200 System (Cell Analysis Systems Inc, Elmhurst, Ill), with normal and hyperplastic glands serving as controls for diploid DNA content. In four cases, the cells of both lesions (PIN and invasive carcinoma) contained only diploid DNA; in one case, an additional aneuploid tumor stem line was present only in the invasive component; and in two cases, both PIN and carcinoma cells contained aneuploid cells having similar DNA content. The concordance of DNA content between PIN and invasive carcinoma in these cases provides evidence that PIN is a precursor lesion for invasive carcinoma.
据推测，前列腺上皮内瘤变（PIN）是前列腺浸润癌的前驱病变，但几乎没有直接证据证明这种关系。因此，我们研究了7例伴有浸润癌的PIN病变上皮细胞的DNA含量。使用CAS200系统（细胞分析系统公司，伊利诺伊州埃尔姆赫斯特）对福尔根染色组织切片中的细胞核DNA含量进行检测，以正常和增生腺体作为二倍体DNA含量的对照。在4例中，两种病变（PIN和浸润癌）的细胞均仅含有二倍体DNA；在1例中，额外的非整倍体肿瘤干细胞系仅存在于浸润成分中；在2例中，PIN和癌细胞均含有DNA含量相似的非整倍体细胞。这些病例中PIN和浸润癌之间DNA含量的一致性为PIN是浸润癌的前驱病变提供了证据。
