Merrall N W, Plevin R J, Stokoe D, Cohen P, Nebreda A R, Gould G W
Department of Biochemistry, University of Glasgow, U.K.
Biochem J. 1993 Oct 15;295 ( Pt 2)(Pt 2):351-5. doi: 10.1042/bj2950351.
Mitogens and growth factors acutely stimulate glucose transport in all cells to supply energy for their growth and division, but little is known about the signalling mechanism by which these agonists promote sugar uptake. Here we show that the transport of deoxyglucose and 3-O-methylglucose into Xenopus laevis oocytes is stimulated about 2.5-fold when mitogen-activated protein kinase (MAP kinase) is microinjected into these oocytes. We also demonstrate that microinjection of the proto-oncogene product c-Mos (an activator of MAP kinase kinase, which activates MAP kinase in Xenopus oocytes), and purified MAP kinase kinase produce similar increases in deoxyglucose transport. Since the activation of MAP kinase is a general response to almost all mitogens and growth factors, we propose that one of its downstream effects is the stimulation of glucose-transport activity.
有丝分裂原和生长因子能迅速刺激所有细胞中的葡萄糖转运，为细胞生长和分裂提供能量，但对于这些激动剂促进糖摄取的信号传导机制却知之甚少。在此我们表明，当将丝裂原活化蛋白激酶（MAP激酶）显微注射到非洲爪蟾卵母细胞中时，脱氧葡萄糖和3 - O - 甲基葡萄糖进入这些卵母细胞的转运受到约2.5倍的刺激。我们还证明，显微注射原癌基因产物c - Mos（MAP激酶激酶的激活剂，在非洲爪蟾卵母细胞中激活MAP激酶）以及纯化的MAP激酶激酶，会使脱氧葡萄糖转运产生类似的增加。由于MAP激酶的激活是对几乎所有有丝分裂原和生长因子的普遍反应，我们提出其下游效应之一是刺激葡萄糖转运活性。
