Lewis L G, Witte D P, Laney D W, Currie M G, Cohen M B
Division of Pediatric Gastroenterology, Children's Hospital Medical Center, Cincinnati, OH 45229.
Biochem Biophys Res Commun. 1993 Oct 29;196(2):553-60. doi: 10.1006/bbrc.1993.2285.
Guanylin, and endogenous ligand for the Escherichia coli heat-stable enterotoxin receptor, is a recently characterized intestinal peptide. To understand the possible physiologic function of guanylin, we examined the cellular location of guanylin mRNA expression in the rat intestine. Intestinal cells were sequentially isolated from villous tip to crypt in rat jejunum and ileum. Northern blots of total RNA identified a single 0.65 kb guanylin transcript predominantly in the villous cell fractions. In situ hybridization studies demonstrated maximal signal intensity in villous cells in rat ileum and surface epithelial cells in the colon. In the ileum, the signal was nonuniform in distribution in the surface epithelial cells, with focal areas of intense signal in clusters of columnar absorptive cells. In both colon and ileum, signal intensity was near background level in deep crypt cells, lamina propria, and muscularis.
鸟苷素是一种最近才被鉴定出来的肠道肽，它是大肠杆菌热稳定肠毒素受体的内源性配体。为了了解鸟苷素可能的生理功能，我们检测了大鼠肠道中鸟苷素mRNA表达的细胞定位。从大鼠空肠和回肠的绒毛顶端到隐窝依次分离肠道细胞。总RNA的Northern印迹分析在绒毛细胞组分中鉴定出一条单一的0.65 kb鸟苷素转录本。原位杂交研究表明，大鼠回肠绒毛细胞和结肠表面上皮细胞中的信号强度最大。在回肠中，表面上皮细胞中的信号分布不均匀，在柱状吸收细胞簇中有信号强烈的局部区域。在结肠和回肠中，深部隐窝细胞、固有层和肌层中的信号强度接近背景水平。
