Piva R, Bianchi N, Aguiari G L, Gambari R, del Senno L
Istituto di Chimica Biologica, Università degli Studi di Ferrara, Italy.
J Steroid Biochem Mol Biol. 1993 Nov;46(5):531-8. doi: 10.1016/0960-0760(93)90179-z.
Two transcripts of the human estrogen receptor (ER) gene have been described, ER mRNA 1 and mRNA 2, different in their 5' untranslated region. By performing reverse transcriptase-polymerase chain reaction with oligonucleotides specific for the 5' genomic region of the human ER gene we have identified a new ER RNA transcript. The sequence analysis of cDNA from MCF7 breast cancer cells and endometrial human tissues demonstrates that this transcript originates further upstream of the initiation transcription sites so far proposed. Primer extension analysis on RNA from MCF7 cells reveals in the upstream region a possible transcription start site at -3090. In agreement with this result, Northern blot analysis shows, in addition to the canonical 6.3 kb ER mRNA, an ER RNA transcript of approx. 7.4 kb in size. The presence of the additional ER mRNA suggests the existence of a new upstream 5' promoter directing transcription of the human ER gene.
已描述了人类雌激素受体（ER）基因的两种转录本，即ER mRNA 1和mRNA 2，它们在5'非翻译区有所不同。通过使用针对人类ER基因5'基因组区域的寡核苷酸进行逆转录聚合酶链反应，我们鉴定出一种新的ER RNA转录本。来自MCF7乳腺癌细胞和人类子宫内膜组织的cDNA序列分析表明，该转录本起源于迄今所提出的起始转录位点的更上游。对MCF7细胞RNA进行的引物延伸分析在上游区域揭示了一个可能位于-3090的转录起始位点。与该结果一致，Northern印迹分析显示，除了典型的6.3 kb ER mRNA外，还有一种大小约为7.4 kb的ER RNA转录本。额外的ER mRNA的存在表明存在一个新的上游5'启动子指导人类ER基因的转录。
