Juneja R K, Gahne B, Sandberg K
Anim Blood Groups Biochem Genet. 1978;9(1):29-36. doi: 10.1111/j.1365-2052.1978.tb01408.x.
Horizontal polyacrylamide gel electrophoreses, on 10% separation gel, of horse serum revealed polymorphism of the vitamin D binding protein (Gc protein) and another post-albumin protein (Pa). Family data supported the hypothesis that Gc and Pa types were controlled by autosomal codominant alleles. For both Gc and Pa proteins, the homozygous types showed a single fraction while the heterozygous type had two fractions. Pa types were found to be identical to the post-albumin types reported earlier by starch gel electrophoresis. Two Gc alleles, GcF and GcS, and three Pa alleles, Pa D, Pa F and Pa S, were observed in samples from Swedish (four breeds), Lipizzaner and Arab horses. The frequency of the more common allele at the two loci, i.e. GcF and PaF, ranged from 0.72-0.93 and from 0.58-0.99, respectively, in the different breeds studied. Plasma samples showed an extra protein fraction near the GcS fraction and thus were found unsuitable for Gc typing.
对马血清在10%分离胶上进行水平聚丙烯酰胺凝胶电泳，结果显示维生素D结合蛋白（Gc蛋白）和另一种后白蛋白蛋白（Pa）具有多态性。家系数据支持以下假说：Gc和Pa类型由常染色体共显性等位基因控制。对于Gc和Pa蛋白，纯合类型显示为单一区带，而杂合类型有两个区带。发现Pa类型与早期通过淀粉凝胶电泳报道的后白蛋白类型相同。在瑞典马（四个品种）、利皮扎马和阿拉伯马的样本中观察到两个Gc等位基因，即GcF和GcS，以及三个Pa等位基因，即Pa D、Pa F和Pa S。在研究的不同品种中，两个位点上较常见等位基因（即GcF和PaF）的频率分别为0.72 - 0.93和0.58 - 0.99。血浆样本在GcS区带附近显示出一个额外的蛋白区带，因此发现其不适用于Gc分型。
