Galson D L, Tan C C, Ratcliffe P J, Bunn H F
Division of Hematology-Oncology, Brigham and Women's Hospital, Boston, MA 02115.
Blood. 1993 Dec 1;82(11):3321-6.
Considerable insights into important cis regulatory elements in a gene can be gleaned from the identification of sequence homologies among different species. To extend and optimize the sequence comparison between human and mouse erythropoietin (Epo) genes, we have obtained new human sequence from 5,547 to 385 bp upstream of the cap site and extended the 3' flank by 489 bp. In addition, we have obtained new sequence information on the mouse Epo gene extending from within the 3' untranslated region (UTR) to 1,001 bp downstream of the polyadenylation site. Analysis of these additional sequences shows considerable homology between human and mouse Epo genes as far as 4 kb (human) or 3 kb (mouse) upstream of the cap sites, as well as far more homology at the 3' end than was previously realized. In addition, both species were found to have a high frequency of short interspersed (SINE) repetitive sequences that interrupt homologies in both the 5' flank and within the transcription unit.
通过鉴定不同物种间的序列同源性，可以深入了解基因中重要的顺式调控元件。为了扩展和优化人与小鼠促红细胞生成素（Epo）基因之间的序列比较，我们获得了新的人类序列，该序列位于帽位点上游5547至385 bp处，并将3'侧翼延伸了489 bp。此外，我们还获得了小鼠Epo基因的新序列信息，该序列从3'非翻译区（UTR）内部延伸至聚腺苷酸化位点下游1001 bp处。对这些额外序列的分析表明，人与小鼠Epo基因在帽位点上游4 kb（人类）或3 kb（小鼠）范围内具有相当高的同源性，并且在3'端的同源性比之前认识到的要高得多。此外，发现两个物种都有高频的短散在（SINE）重复序列，这些序列中断了5'侧翼和转录单元内的同源性。
