Lorenzato M, Brabencova E, Visseaux-Coletto B, Prevoteau L, Adnet J J
Laboratoire POL BOUIN, CHU Maison Blanche 45, Reims.
Bull Assoc Anat (Nancy). 1993 Mar;77(236):9-14.
There are many ways to analyse and quantify the proliferating activity of a tumor. The different technics are more and more specific of each phase of the cell cycle. This paper firstly describes the basis of each of these technics (mitosis count, DNA staining with various compounds fluorescent or not, incorporation of specific molecules during the phase of DNA synthesis, detection of proliferation antigens by immunological methods). Secondly, the interest of the proliferating fraction knowledge of a tumor is discussed, for various pathologies and according to the technic used.
有许多方法可用于分析和量化肿瘤的增殖活性。不同的技术对细胞周期的每个阶段越来越具有特异性。本文首先描述了这些技术中的每一种的基础（有丝分裂计数、用各种荧光或非荧光化合物进行DNA染色、在DNA合成阶段掺入特定分子、通过免疫方法检测增殖抗原）。其次，针对各种病理学情况并根据所使用的技术，讨论了了解肿瘤增殖分数的意义。
