Levite M, Zinger H, Mozes E, Reisner Y
Department of Membrane Research and Biophysics, Weizmann Institute of Science, Rehovot, Israel.
Bone Marrow Transplant. 1993 Sep;12(3):179-83.
Experimental systemic lupus erythematosus (SLE) can be induced in mice by immunization with either a human monoclonal anti-DNA antibody bearing the 16/6 idiotype (16/6 Id) or with a mouse monoclonal anti-idiotypic antibody specific for the 16/6 Id. Susceptibility to the induction of experimental SLE is genetically determined but is not linked to the MHC. In the present study we tested the susceptibility of BM chimeras of different donor-host combinations to the induction of SLE and found that high levels of anti-16/6 Id and anti-ssDNA antibodies were induced in BALB/c-->C57BL/6, BALB/c-->BALB/c and normal BALB/c mice as opposed to C57BL/6-->BALB/c chimeras and normal C57BL/6 mice. The low levels of the anti-16/6 Id and anti-ssDNA antibodies produced by C57BL/6-->BALB/c chimeras immunized with the 16/6 fully allogeneic BMT as such chimeras were shown to produce high levels of antibodies to a T cell-dependent antigen (the synthetic polypeptide (Phe,G)-A--L). These results demonstrate that the production of SLE-related autoantibodies is controlled by donor-type BM derived cells and not by host-type cells in the thymic stroma.
实验性系统性红斑狼疮（SLE）可通过用携带16/6独特型（16/6 Id）的人单克隆抗DNA抗体或用对16/6 Id特异的小鼠单克隆抗独特型抗体免疫小鼠来诱导。对实验性SLE诱导的易感性是由基因决定的，但与主要组织相容性复合体（MHC）无关。在本研究中，我们测试了不同供体-宿主组合的骨髓嵌合体对SLE诱导的易感性，发现与C57BL/6→BALB/c嵌合体和正常C57BL/6小鼠相反，在BALB/c→C57BL/6、BALB/c→BALB/c和正常BALB/c小鼠中诱导出了高水平的抗16/6 Id和抗单链DNA（ssDNA）抗体。用16/6完全同种异体骨髓移植（BMT）免疫的C57BL/6→BALB/c嵌合体产生的抗16/6 Id和抗ssDNA抗体水平较低，因为这类嵌合体被证明能产生高水平的针对T细胞依赖性抗原（合成多肽（Phe，G）-A--L）的抗体。这些结果表明，SLE相关自身抗体的产生是由供体型骨髓来源的细胞控制的，而不是由胸腺基质中的宿主型细胞控制的。
