通过亲和层析法纯化人血浆激肽释放酶和尿激酶。

Purification of human plasma kallikrein and urokinase by affinity chromatography.

作者信息

Tamura Y, Fujii S

出版信息

J Biochem. 1976 Sep;80(3):507-11. doi: 10.1093/oxfordjournals.jbchem.a131305.

DOI:10.1093/oxfordjournals.jbchem.a131305

Abstract

L-Homoarginine benzylester was coupled with Sepharose 4B and used for affinity chromatography of human plasma kallikrein [EC 3.4.21.8] and urokinase [EC 3.4.99.26]. The euglobulin fraction of human plasma was treated with disodium ethylenediamine-tetraacetate (EDTA) and activated with acetone, and then kallikrein was purified 16-fold by affinity chromatography. The yield of kallikrein activity on affinity chromatography was 53%. The purified preparation of human plasma kallikrein appeared to be homogenous on gel electrophoresis. Commercial urokinase was also purified 4.8-fold with 64% yield by affinity chromatography.

摘要

L-高精氨酸苄酯与琼脂糖4B偶联，并用于人血浆激肽释放酶[EC 3.4.21.8]和尿激酶[EC 3.4.99.26]的亲和层析。人血浆的优球蛋白部分用乙二胺四乙酸二钠（EDTA）处理并用丙酮活化，然后通过亲和层析将激肽释放酶纯化16倍。亲和层析中激肽释放酶活性的产率为53%。人血浆激肽释放酶的纯化制剂在凝胶电泳上似乎是均一的。市售尿激酶也通过亲和层析纯化了4.8倍，产率为64%。