Tan D M, Hu G L, Arima T, Zhang Z, Nanba T, Hatanaka T
Department of Infectious Diseases, First Affiliated Hospital, Hunan Medical University, Changsha.
Chin Med J (Engl). 1993 Jul;106(7):522-6.
Fragments of core and NS3 of hepatitis C virus-Hunan (HCV-Hun) were cloned by RT-PCR and gene recombinant techniques from blood samples collected in Hunan Province, China. In comparison with sequences of our samples with those of HCV-US and HCV-J, the homologies of nucleotides and amino acids were about 90%, indicating that fragments of core and NS3 of HCV-Hun were in a relative conserved region of HCV. Two fusion proteins containing the peptides coded by HCV core (MBP-HCV core) and HCV. NS3 (MBP-HCV. NS3-Gal) were expressed by Escherichia Coli with recombinant plasmids. The specific HCV antigenicity of the two fusion proteins were identified by western blotting. Therefore, MBP-HCV. core and MBP-HCV.NS3-Gal were found useful for anti-HCV assay.
通过逆转录聚合酶链反应（RT-PCR）和基因重组技术，从中国湖南省采集的血液样本中克隆出丙型肝炎病毒-湖南株（HCV-Hun）的核心片段和NS3片段。将我们样本的序列与HCV-US和HCV-J的序列进行比较，核苷酸和氨基酸的同源性约为90%，表明HCV-Hun的核心片段和NS3片段处于HCV的相对保守区域。用重组质粒通过大肠杆菌表达了两种包含由HCV核心编码的肽（MBP-HCV核心）和HCV NS3（MBP-HCV NS3-Gal）的融合蛋白。通过蛋白质印迹法鉴定了这两种融合蛋白的特异性HCV抗原性。因此，发现MBP-HCV核心和MBP-HCV NS3-Gal可用于抗HCV检测。
