Xu Z C
Beijing General Hospital, PLA.
Zhonghua Xin Xue Guan Bing Za Zhi. 1993 Jun;21(3):173-5, 188.
Thirty male N-Z white rabbits were randomized into 3 groups (10 each). Normal control group (C) were fed regular chow. Atherosclerotic control group (CAS) were fed a chow containing 1% cholesterol. Nifedipine group (N) were fed 1% cholesterol chow and nifedipine 40 mg/animal/day. The experiment lasted for 12 weeks. Blood total cholesterol, HDL-C, MDA, 6-keto-PGF1 alpha and TXB2 were measured. After the sacrifice of the animals, electron probe microanalysis was employed to cryo-ultrathin sections of the aorta for measuring cytoplasmic calcium content in situ in the medial smooth muscle cells. The area of atherosclerotic plaques of the aortae was planimetried with computer. The results showed that MDA and TXB2 in group CAS were markedly higher than those in groups C and N (P < 0.01 or 0.05). The cytoplasmic calcium in group CAS was significantly higher than that in groups C and N (P < 0.01). The difference between groups C and N was not significant. The area of atherosclerotic lesions in group CAS was 44 +/- 8%, markedly larger than that in group N (13 +/- 5%, P < 0.01). The antiatherosclerotic effects of nifedipine may be mainly by a mechanism of inhibiting intracellular calcium overload.
将30只雄性新西兰白兔随机分为3组（每组10只）。正常对照组（C组）给予常规饲料。动脉粥样硬化对照组（CAS组）给予含1%胆固醇的饲料。硝苯地平组（N组）给予含1%胆固醇的饲料及硝苯地平40mg/只/天。实验持续12周。检测血液总胆固醇、高密度脂蛋白胆固醇、丙二醛、6-酮-前列腺素F1α和血栓素B2。动物处死后，采用电子探针微分析法对主动脉冷冻超薄切片进行检测，以原位测定中膜平滑肌细胞的胞质钙含量。用计算机测量主动脉粥样硬化斑块面积。结果显示，CAS组的丙二醛和血栓素B2明显高于C组和N组（P<0.01或0.05）。CAS组的胞质钙明显高于C组和N组（P<0.01）。C组和N组之间差异无统计学意义。CAS组的动脉粥样硬化病变面积为44±8%，明显大于N组（13±5%，P<0.01）。硝苯地平的抗动脉粥样硬化作用可能主要是通过抑制细胞内钙超载的机制实现的。
