Salovsky P, Shopova V, Dancheva V, Marev R, Pandurska A
Department of Disaster Medicine, University of Medicine, Pleven, Bulgaria.
Environ Health Perspect. 1993 Jul;101 Suppl 2(Suppl 2):269-74. doi: 10.1289/ehp.93101s2269.
Experiments involving 120 male Wistar rats were performed to study the effect of treatment with cadmium acetate and external irradiation. A single 0.5 mg/kg body weight dose of cadmium acetate was administered intratracheally. Shortly thereafter, the animals received a single whole-body exposure to 4 Gy gamma rays (cesium source). Findings indicated the chemical elevated enzyme activities of lactate dehydrogenase (LDH), alkaline phosphatase (AlP), and acid phosphatase (AP), as well as protein content and percentage of neutrophils in bronchoalveolar lavage fluid (BALF); the percentage of alveolar macrophages was sharply reduced. Radiation alone produced no substantial changes in the parameters investigated. Treatment with both agents combined was found to result in a synergistic rise of LDH, AlP, and AP activities and protein content in BALF. It was concluded that the BALF biochemical markers used are reliable indicators for identifying the type of combined effect produced in the lungs by chemical agents and ionizing radiation.
进行了涉及120只雄性Wistar大鼠的实验，以研究醋酸镉处理和外部照射的影响。经气管内给予单次剂量为0.5毫克/千克体重的醋酸镉。此后不久，动物接受单次全身4 Gyγ射线（铯源）照射。结果表明，该化学物质提高了支气管肺泡灌洗液（BALF）中乳酸脱氢酶（LDH）、碱性磷酸酶（AlP）和酸性磷酸酶（AP）的酶活性，以及蛋白质含量和中性粒细胞百分比；肺泡巨噬细胞百分比急剧下降。单独照射对所研究的参数没有产生实质性变化。发现两种试剂联合处理导致BALF中LDH、AlP和AP活性以及蛋白质含量协同升高。得出的结论是，所使用的BALF生化标志物是识别化学试剂和电离辐射在肺部产生的联合效应类型的可靠指标。
