Katagiri S, Takamatsu T, Minamikawa T, Fujita S
Department of Pathology, Kyoto Prefectural University of Medicine, Japan.
FEBS Lett. 1993 Nov 22;334(3):343-6. doi: 10.1016/0014-5793(93)80708-3.
To clarify the mechanism of secretagogue (compound 48/80)-induced calcium signaling in rat peritoneal mast cells, we analyzed serial confocal calcium images with high spatial and temporal resolution using different Ca(2+)-probes. The Ca(2+)-wave began at the periphery of the cytoplasm, and then spread to the center of the nucleus. Nuclear [Ca2+]i was clearly higher than cytoplasmic [Ca2+]i. The heterogeneity of [Ca2+]i continued until about 2 min after degranulation. These results suggest the existence of an intranuclear Ca(2+)-store which possesses a Ca(2+)-releasing mechanism similar to that in the cytoplasm.
为阐明促分泌素（化合物48/80）诱导大鼠腹膜肥大细胞钙信号传导的机制，我们使用不同的Ca(2+)探针，以高空间和时间分辨率分析了连续共聚焦钙图像。Ca(2+)波从细胞质周边开始，然后扩散到细胞核中心。细胞核内的[Ca2+]i明显高于细胞质内的[Ca2+]i。[Ca2+]i的异质性一直持续到脱颗粒后约2分钟。这些结果表明存在一种核内钙库，其具有与细胞质中类似的钙释放机制。
