Goldman K N, Centifanto Y, Kaufman H E, Slappey T E
Arch Ophthalmol. 1978 Dec;96(12):2277-80. doi: 10.1001/archopht.1978.03910060573023.
A simple method has been developed to reduce contamination in postmortem donor human eyes in anticipation of corneal transplantation. In vivo investigation of albino rabbits demonstrates that vigorous saline solution irrigation is extremely effective in decreasing the surface bacterial counts of the postmortem eye. In vitro and in vivo studies show that Neosporin kills bacteria at room temperature and further show that a tenfold increase in the thimerosal concentration of the Neosporin will kill fungus. Postmortem eyes contaminated by pathogenic organisms can be effectively cleaned by a combination of saline solution irrigation and the new Neosporin-thimerosal solution. No substantial damage of the donor tissue was noted by scanning electron microscopy. Human eyes cultured before this procedure were all contaminated, but after cleansing and immersion, no bacterial or fungal growth occurred.
已经开发出一种简单的方法来减少用于角膜移植的死后供体人眼的污染。对白化兔的体内研究表明，强力生理盐水冲洗在降低死后眼睛表面细菌数量方面极为有效。体外和体内研究表明，新霉素在室温下能杀死细菌，并且进一步表明新霉素中硫柳汞浓度增加十倍将杀死真菌。被致病生物污染的死后眼睛可以通过生理盐水冲洗和新的新霉素 - 硫柳汞溶液联合有效地清洁。扫描电子显微镜未发现供体组织有实质性损伤。在此操作之前培养的人眼均被污染，但在清洁和浸泡后，未出现细菌或真菌生长。
