Tajima F
Department of Population Genetics, National Institute of Genetics, Shizuoka-ken, Japan.
Genetics. 1993 Oct;135(2):599-607. doi: 10.1093/genetics/135.2.599.
Simple statistical methods for testing the molecular evolutionary clock hypothesis are developed which can be applied to both nucleotide and amino acid sequences. These methods are based on the chi-square test and are applicable even when the pattern of substitution rates is unknown and/or the substitution rate varies among different sites. Furthermore, some of the methods can be applied even when the outgroup is unknown. Using computer simulations, these methods were compared with the likelihood ratio test and the relative rate test. The results indicate that the powers of the present methods are similar to those of the likelihood ratio test and the relative rate test, in spite of the fact that the latter two tests assume that the pattern of substitution rates follows a certain model and that the substitution rate is the same among different sites, while such assumptions are not necessary to apply the present methods. Therefore, the present methods might be useful.
开发了用于检验分子进化钟假说的简单统计方法，这些方法可应用于核苷酸序列和氨基酸序列。这些方法基于卡方检验，即使在替代率模式未知和/或不同位点的替代率不同时也适用。此外，即使外类群未知，其中一些方法也可应用。通过计算机模拟，将这些方法与似然比检验和相对速率检验进行了比较。结果表明，尽管后两种检验假设替代率模式遵循某种模型且不同位点的替代率相同，但本方法的功效与似然比检验和相对速率检验相似，而应用本方法则无需此类假设。因此，本方法可能有用。
