来自耐热紫色链霉菌OPC-520的耐热几丁质酶编码基因的克隆与序列分析。
Cloning and sequence analysis of the gene encoding a thermostable chitinase from Streptomyces thermoviolaceus OPC-520.
作者信息
Tsujibo H, Endo H, Minoura K, Miyamoto K, Inamori Y
机构信息
Osaka University of Pharmaceutical Sciences, Japan.
出版信息
Gene. 1993 Nov 30;134(1):113-7. doi: 10.1016/0378-1119(93)90183-4.
The gene (chi40) encoding a thermostable chitinase from Streptomyces thermoviolaceus OPC-520 was cloned in Escherichia coli JM109 using pUC18. The nucleotide (nt) sequence of chi40 has been determined. A single open reading frame (ORF) encoded a protein consisting of 414 amino acids (aa) with a M(r) of 43,838. The deduced aa sequence of the cloned chitinase (Chi40) showed striking homology (74%) with Chi63 from Streptomyces plicatus. Comparison with other chitinases revealed that Chi40 contained the two conserved regions common to microbial and plant chitinases. Furthermore, the putative promoter region of chi40, which might be involved in the repression and induction of such catabolite-controlled genes, was detected by the DNA sequence alignments.
利用pUC18将编码来自嗜热紫链霉菌OPC - 520的一种耐热几丁质酶的基因（chi40）克隆到大肠杆菌JM109中。已确定chi40的核苷酸（nt）序列。一个单一的开放阅读框（ORF）编码一个由414个氨基酸（aa）组成、分子量为43,838的蛋白质。克隆的几丁质酶（Chi40）推导的氨基酸序列与褶皱链霉菌的Chi63显示出显著的同源性（74%）。与其他几丁质酶比较发现，Chi40含有微生物和植物几丁质酶共有的两个保守区域。此外，通过DNA序列比对检测到chi40可能参与此类分解代谢物控制基因的阻遏和诱导的假定启动子区域。
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