链霉菌纤维素酶（纤维二糖酶）合成的生理学研究。
Physiological Studies of Cellulase (Avicelase) Synthesis in Streptomyces reticuli.
出版信息
Appl Environ Microbiol. 1996 Mar;62(3):1065-9. doi: 10.1128/aem.62.3.1065-1069.1996.
Abstract
Cellulase (Avicelase, Cel1) from Streptomyces reticuli efficiently hydrolyzes crystalline cellulose (Avicel) to cellobiose. The synthesis of the enzyme was found to be dependent on the presence of insoluble Avicel but not on either soluble hydroxyethylcellulose, cellooligomers, or cellobiose. Glycerol and various metabolizable mono- and disaccharides repress Avicelase synthesis, whereas yeast extract has no inducing or repressing effect. Glucose kinase is not required for the repression effect. In the course of cultivation, S. reticuli secretes significant quantities of acid, predominantly pyruvate and succinate, which reduce the pH to 4 in commonly used media with low buffering capacity. Comparative studies with media with low and high buffering capacities revealed that Avicelase synthesis is strongly repressed at a low pH.
摘要
来自链霉菌属的纤维素酶（纤维二糖酶，Cel1）能够有效地将结晶纤维素（纤维素）水解为纤维二糖。研究发现，该酶的合成依赖于不溶性纤维素的存在，但不依赖于可溶性羟乙基纤维素、低聚糖或纤维二糖。甘油和各种可代谢的单糖和二糖抑制纤维二糖酶的合成，而酵母提取物对其没有诱导或抑制作用。葡萄糖激酶不是抑制作用所必需的。在培养过程中，链霉菌属分泌大量的酸，主要是丙酮酸和琥珀酸，这会使低缓冲能力的常用培养基的 pH 值降低到 4。与低缓冲能力和高缓冲能力的培养基的比较研究表明，在低 pH 值下，纤维二糖酶的合成受到强烈抑制。
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