普通变形杆菌mutT基因的序列及特性分析

Sequence and characterization of mutT from Proteus vulgaris.

作者信息

Kamath A V, Yanofsky C

机构信息

Department of Biological Sciences, Stanford University, CA 94305-5020.

出版信息

Gene. 1993 Nov 30;134(1):99-102. doi: 10.1016/0378-1119(93)90180-b.

DOI:10.1016/0378-1119(93)90180-b

PMID:8244038

Abstract

A cloned DNA fragment containing the tryptophanase (tna) operon of Proteus vulgaris was found to contain a gene analogous to mutT of Escherichia coli immediately distal to the tna operon. The presumptive mutT of P. vulgaris was shown to be a functional gene by complementation of a mutT mutant from E. coli. The deduced amino acid sequence of the MutT polypeptide of P. vulgaris was 47% identical and 70% similar to MutT of E. coli. The mutT and tna operons of P. vulgaris were shown to be adjacent on the genome of this organism. These operons are located about 20 min apart in the E. coli genome. Our findings suggest that either or both tna and mutT have different genomic locations in the two organisms.

摘要

发现一个含有普通变形杆菌色氨酸酶（tna）操纵子的克隆DNA片段，在tna操纵子紧邻的下游含有一个与大肠杆菌mutT类似的基因。通过对大肠杆菌mutT突变体的互补作用，证明普通变形杆菌的假定mutT是一个功能基因。普通变形杆菌MutT多肽的推导氨基酸序列与大肠杆菌MutT的一致性为47%，相似性为70%。普通变形杆菌的mutT和tna操纵子在该生物体的基因组上相邻。在大肠杆菌基因组中，这些操纵子相距约20分钟。我们的研究结果表明，tna和mutT中的一个或两个在这两种生物体中具有不同的基因组位置。