Akiyama M, Horiuchi T, Sekiguchi M
Mol Gen Genet. 1987 Jan;206(1):9-16. doi: 10.1007/BF00326530.
The Escherichia coli mutator gene mutT, which causes A:T----C:G transversion, was cloned in pBR 322. mutT+ plasmids carry a 0.9 kb PvuII DNA fragment derived from the E. coli chromosome. Specific labelling of plasmid-encoded proteins by the maxicell method revealed that mutT codes for a polypeptide of about 15,000 daltons. The protein was overproduced when the mutT gene was placed under the control of the lac regulatory region on a multicopy runaway plasmid. The nucleotide sequence of the mutT gene was determined by the dideoxy method.
可引起A:T到C:G颠换的大肠杆菌诱变基因mutT被克隆到pBR 322中。mutT⁺质粒携带一个源自大肠杆菌染色体的0.9 kb PvuII DNA片段。通过大细胞法对质粒编码蛋白进行特异性标记显示，mutT编码一种约15,000道尔顿的多肽。当mutT基因置于多拷贝失控质粒上的lac调控区控制之下时，该蛋白质会过量产生。mutT基因的核苷酸序列通过双脱氧法测定。
