Greenwood M R, Tasker L, Cobbold P H, Marshall-Clarke S
Department of Human Anatomy and Cell Biology, University of Liverpool, U.K.
Immunology. 1993 Sep;80(1):51-5.
Cross-linking of surface-immunoglobulin (sIg) has been associated with IP3 production and a rise in cytoplasmic-free calcium ([Ca2+]i) in studies of populations of normal and transformed B cells. We have examined the kinetics of the induced cytoplasmic calcium rises in single, Fura-2-loaded cells, during stimulation with a variety of agonists. Our data indicate that the responses of B cells to some stimuli, such as elevated cyclic AMP, consist of repetitive calcium transients, but these Ca2+ oscillations do not occur after sIg ligation. Rather, sIg cross-linking leads to a rapid rise in cytoplasmic calcium which is followed by a sustained elevation. Most of this response seems to result from an inflow of extracellular calcium rather than from internal stores.
在对正常和转化B细胞群体的研究中，表面免疫球蛋白（sIg）的交联与肌醇三磷酸（IP3）的产生以及细胞质游离钙（[Ca2+]i）的升高有关。我们已经研究了在用各种激动剂刺激期间，单个负载Fura-2的细胞中诱导的细胞质钙升高的动力学。我们的数据表明，B细胞对某些刺激（如环磷酸腺苷升高）的反应由重复性钙瞬变组成，但在sIg连接后不会出现这些Ca2+振荡。相反，sIg交联导致细胞质钙迅速升高，随后持续升高。这种反应的大部分似乎是由细胞外钙流入引起的，而不是来自内部储存。
