Randle E S, Waanders G A, Masciantonio M, Godfrey D I, Boyd R L
Department of Pathology and Immunology, Monash University Medical School, Prahran, Australia.
J Immunol. 1993 Dec 1;151(11):6027-35.
Previously, we detailed the characterization of thymic shared Ag-1, a unique marker of immature thymocytes and isolated thymic stromal cells, defined by the mAb MTS 35. In this study, the functional relevance of this molecule to thymopoiesis was investigated by the addition of purified MTS 35 to fetal thymus organ culture. It down-regulated thymic shared Ag-1 expression and dramatically reduced thymocyte cell yield through inhibition of alpha beta-TcR+ T cell differentiation, post CD3-CD4-CD8- triple negative thymocytes. These effects were specific for the mAb MTS 35, because controls, which include both isotype-matched and other lymphostromal mAb, showed no similar effects. These results demonstrate that thymic shared Ag-1 is a functionally important marker of early thymocyte differentiation, particularly with regard to the alpha beta-TcR lineage.
此前，我们详细描述了胸腺共享抗原-1的特征，它是未成熟胸腺细胞和分离的胸腺基质细胞的独特标志物，由单克隆抗体MTS 35定义。在本研究中，通过向胎胸腺器官培养物中添加纯化的MTS 35，研究了该分子与胸腺生成的功能相关性。它下调了胸腺共享抗原-1的表达，并通过抑制αβ-TcR + T细胞分化，即CD3-CD4-CD8-三阴性胸腺细胞之后的分化，显著降低了胸腺细胞产量。这些效应对于单克隆抗体MTS 35是特异性的，因为包括同型匹配和其他淋巴基质单克隆抗体在内的对照均未显示出类似效应。这些结果表明，胸腺共享抗原-1是早期胸腺细胞分化的功能重要标志物，特别是对于αβ-TcR谱系而言。
