Appasamy P M, Kenniston T W, Weng Y, Holt E C, Kost J, Chambers W H
Pittsburgh Cancer Institute, Pennsylvania 15213.
J Exp Med. 1993 Dec 1;178(6):2201-6. doi: 10.1084/jem.178.6.2201.
We previously reported that culture of murine fetal liver (FL) cells with interleukin 7 (IL-7) results in expression of high levels of T cell receptor (TCR) gamma transcripts by a population of cells expressing Thy-1 and Pgp-1, suggesting that IL-7 promotes the growth and/or differentiation of pre-T cells. We demonstrate herein that culture of FL cells for 7 d with IL-7 caused the rearrangement and expression of TCR gamma variable (V) region genes V gamma 4 and V gamma 6, but not V gamma 5 or V gamma 7. Since this effect was not blocked by hydroxyurea, it appeared to represent induction of expression of these genes by IL-7 rather than expansion of a preexisting positive population. We also show that IL-7 induced RAG-1 and RAG-2 mRNA expression by FL cells. These data provide evidence that specific TCR gamma/delta V region genes can be rearranged and expressed by T lineage cells before their migration to the thymus, in response to IL-7.
我们之前报道过，用白细胞介素7（IL-7）培养小鼠胎肝（FL）细胞会导致一群表达Thy-1和Pgp-1的细胞高水平表达T细胞受体（TCR）γ转录本，这表明IL-7促进前T细胞的生长和/或分化。我们在此证明，用IL-7培养FL细胞7天会导致TCRγ可变（V）区基因Vγ4和Vγ6发生重排和表达，但Vγ5或Vγ7不会。由于这种效应未被羟基脲阻断，所以它似乎代表IL-7对这些基因表达的诱导，而不是预先存在的阳性群体的扩增。我们还表明，IL-7诱导FL细胞表达RAG-1和RAG-2 mRNA。这些数据提供了证据，表明特定的TCRγ/δ V区基因在T系细胞迁移到胸腺之前，可响应IL-7发生重排和表达。
