Peterseim D S, Meyers C H, Craig D M, Heinle J S, Jayawant A M, Devaney J J, Klitzman B M, Van Trigt P
Department of Surgery, Duke University Medical Center, Durham, North Carolina 27710.
J Surg Res. 1993 Dec;55(6):588-94. doi: 10.1006/jsre.1993.1189.
The purpose of this study was to determine if oxygen delivery to rabbit cardiac allografts arrested and stored in University of Wisconsin solution (UWS) at 4 degrees C would affect preservation. Nineteen isolated rabbit hearts were rapidly excised and perfused at 80 mm Hg on an isovolumic modified Langendorff apparatus. A micromanometer was placed within a balloon and inserted into the left ventricle through the mitral valve orifice. Digitized pressure waveforms were collected at 11 known balloon volumes from 0.8 to 1.2 ml. Baseline data were obtained for all hearts while perfused with Krebs-Henseleit solution equilibrated with 95% O2:5% CO2 at 37 degrees C. All hearts were arrested with 30 ml of UWS (290 mOsm). The control group (N = 10) was stored in UWS at 4 degrees C for 8 hr, and the experimental group (N = 9) was perfused with oxygenated UWS (O2 content = 5.6 ml O2) at 4 degrees C for 8 hr at a pressure of 60 mm Hg (5-10 ml/min). Both groups were then reperfused with Krebs-Henseleit buffer at 80 mm Hg for 15 min at for postpreservation data acquisition. Left ventricular developed pressures over a physiologic range (pressure-volume area) and maximum positive and negative dP/dt were calculated. Recovery of left ventricular parameters as a percentage of the baseline values was determined. Mean pressure-volume area recovery in the nonperfused group was 40 +/- 7.9% versus the perfused group (71 +/- 7.0%, P = 0.01).(ABSTRACT TRUNCATED AT 250 WORDS)
本研究的目的是确定在4℃下，向用威斯康星大学溶液（UWS）灌注并储存的兔心脏异体移植物输送氧气是否会影响保存效果。19颗离体兔心脏被迅速切除，并在改良的等容Langendorff装置上以80 mmHg进行灌注。将一个微测压计置于球囊内，通过二尖瓣口插入左心室。在0.8至1.2 ml的11个已知球囊容积下采集数字化压力波形。在用37℃下用95% O2:5% CO2平衡的Krebs-Henseleit溶液灌注时，获取所有心脏的基线数据。所有心脏均用30 ml的UWS（290 mOsm）停搏。对照组（N = 10）在4℃下于UWS中储存8小时，实验组（N = 9）在4℃下以60 mmHg（5 - 10 ml/min）的压力用含氧UWS（氧含量 = 5.6 ml O2）灌注8小时。然后两组均在80 mmHg下用Krebs-Henseleit缓冲液再灌注15分钟，以获取保存后的数据。计算生理范围内的左心室舒张末压（压力 - 容积面积）以及最大正负dP/dt。确定左心室参数相对于基线值的恢复百分比。未灌注组的平均压力 - 容积面积恢复率为40 ± 7.9%，而灌注组为71 ± 7.0%，P = 0.01。（摘要截断于250字）
