Collado-Vides J
Centro de Investigación sobre Fijación de Nitrógeno, Universidad Nacional Autónoma de México, Morelos.
J Theor Biol. 1993 Aug 21;163(4):527-48. doi: 10.1006/jtbi.1993.1136.
An integrative approach to the study of the regulation of gene expression has been undertaken here. The main goal of this approach is to make explicit the common rules that govern the relative location of regulatory sites within operons and other units of genetic information (UGIs). A classification that emphasizes the regulatory properties of UGIs can be achieved by partitioning UGIs into short sequences with defined properties. Such a classification scheme can be precisely defined as a Grammar with a component of combinatorial (rewriting) rules, and a dictionary component. Sequences have then to be grouped into classes such that any sequence of the same class can mutually substitute and produce novel regulatable UGIs. It is shown here that individual nucleotides cannot define such classes--they are far from equivalent to phonemes. Neither pairs, triplets or any short sequence with a defined number of nucleotides can define productive substitutions. Defined sequences like promoter, operator and activator binding sites are the smallest elements of combinatorial rules within the defined range of transcription initiation of sigma 70 Escherichia coli promoters.
本文采用了一种综合方法来研究基因表达的调控。这种方法的主要目标是明确支配操纵子和其他遗传信息单元（UGIs）内调控位点相对位置的通用规则。通过将UGIs划分为具有特定属性的短序列，可以实现一种强调UGIs调控特性的分类。这样的分类方案可以精确地定义为一种具有组合（重写）规则组件和字典组件的语法。然后，序列必须被分组到不同的类别中，使得同一类别的任何序列都可以相互替代并产生新的可调控UGIs。本文表明，单个核苷酸无法定义此类类别——它们远不等同于音素。无论是双核苷酸、三核苷酸还是任何具有特定数量核苷酸的短序列都无法定义有效的替代。像启动子、操纵子和激活剂结合位点这样的特定序列是大肠杆菌σ70启动子转录起始定义范围内组合规则的最小元素。
