Dang Y, Lowe G M, Edwards S W, Galvani D W
University Department of Haematology, Royal Liverpool University Teaching Hospital, U.K.
Leuk Res. 1993 Dec;17(12):1037-44. doi: 10.1016/0145-2126(93)90160-m.
When purified control neutrophils were primed with GM-CSF, a significant increase in FMLP-induced MPO release was observed (mean +/- S.E.M., 3.4 +/- 0.8 mU/10(7) unprimed cells compared to 6.5 +/- 1.1 mU/10(7) primed cells, p < 0.001). This MPO release was greatly augmented by Cytochalasin B (Cy B), but after the addition of Cy B the priming effects of GM-CSF became less obvious. Exposure to GM-CSF without FMLP did not enhance MPO release. Within whole blood, FMLP produced negligible MPO release, but priming with GM-CSF prior to FMLP always resulted in a significant increase in MPO release. Myelodysplastic neutrophils released similar amounts of MPO in response to FMLP, compared with control cells (3.4 +/- 0.8 mU/10(7) control cells compared to 2.7 +/- 0.3 mU/10(7) MDS cells, p > 0.05). Priming with GM-CSF produced an increase in FMLP-stimulated MPO release comparable with control cells. In terms of total MPO content, although some MDS patients exhibited low levels, as a group there was no significant difference from controls (169 +/- 21 mU/10(7) control cells compared with 157 +/- 19 mU/10(7) MDS cells). These findings suggest that MPO activity is not a universal defect in MDS and cannot account for the defects in respiratory burst activity in these neutrophils.
当用粒细胞-巨噬细胞集落刺激因子（GM-CSF）预处理纯化的对照中性粒细胞时，观察到甲酰甲硫氨酸-亮氨酸-苯丙氨酸（FMLP）诱导的髓过氧化物酶（MPO）释放显著增加（平均值±标准误，未预处理细胞为3.4±0.8 mU/10⁷个细胞，预处理细胞为6.5±1.1 mU/10⁷个细胞，p<0.001）。细胞松弛素B（Cy B）可极大增强这种MPO释放，但加入Cy B后GM-CSF的预处理作用变得不那么明显。单独暴露于GM-CSF而无FMLP时不会增强MPO释放。在全血中，FMLP引起的MPO释放可忽略不计，但在FMLP之前用GM-CSF预处理总是会导致MPO释放显著增加。与对照细胞相比，骨髓增生异常综合征（MDS）患者的中性粒细胞对FMLP释放的MPO量相似（对照细胞为3.4±0.8 mU/10⁷个细胞，MDS细胞为2.7±0.3 mU/10⁷个细胞，p>0.05）。用GM-CSF预处理可使FMLP刺激的MPO释放增加，与对照细胞相当。就MPO总含量而言，尽管一些MDS患者水平较低，但作为一个整体与对照组无显著差异（对照细胞为169±21 mU/10⁷个细胞，MDS细胞为157±19 mU/10⁷个细胞）。这些发现表明，MPO活性不是MDS中的普遍缺陷，不能解释这些中性粒细胞呼吸爆发活性的缺陷。
