Kinesin-mediated vesicular transport in a biochemically defined assay.
作者信息
Urrutia R, Murphy D B, Kachar B, McNiven M A
机构信息
Laboratory of Cellular Biology, National Institute for Deafness and Other Communication Disorders, National Institutes of Health, Bethesda, Maryland 20892.
出版信息
Methods Cell Biol. 1993;39:253-66. doi: 10.1016/s0091-679x(08)60175-2.
PMID:8246802
Abstract
Here we have described simple and reproducible methods to observe kinesin-mediated vesicle and microtubule movements under defined conditions using video microscopy. We are optimistic that this assay will provide a useful tool to study kinesin function, regulation, and dynamic physical interactions with membranous organelles and microtubules.
摘要
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