Urrutia R, Murphy D B, Kachar B, McNiven M A
Laboratory of Cellular Biology, National Institute for Deafness and Other Communication Disorders, National Institutes of Health, Bethesda, Maryland 20892.
Methods Cell Biol. 1993;39:253-66. doi: 10.1016/s0091-679x(08)60175-2.
Here we have described simple and reproducible methods to observe kinesin-mediated vesicle and microtubule movements under defined conditions using video microscopy. We are optimistic that this assay will provide a useful tool to study kinesin function, regulation, and dynamic physical interactions with membranous organelles and microtubules.
