用于嘧啶光二聚体特异性抗体的VH和VL基因的克隆。
Cloning of VH and VL genes for antibodies specific for pyrimidine photo dimers.
作者信息
Morioka H, Miura H, Kamiya H, Matsunaga T, Nikaido O, Ohtsuka E
机构信息
Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
出版信息
Nucleic Acids Symp Ser. 1993(29):111-2.
We have prepared two types of cDNAs encoding mouse immunoglobulin heavy (VH) and light (VL) chain variable (V) domains against UV-induced DNA damage by the polymerase chain reaction (PCR). The amplified cDNA was cloned for sequencing and expression, and the nucleotide sequences of the V domains were determined. The deduced amino acid sequences of the complementarity-determining regions (CDRs) indicated that CDR3 of the VH domain and CDR2 of the VL domain might be concerned with the recognition of photolesions.
我们通过聚合酶链反应（PCR）制备了两种编码小鼠免疫球蛋白重链（VH）和轻链（VL）可变（V）结构域的cDNA，用于对抗紫外线诱导的DNA损伤。扩增的cDNA被克隆用于测序和表达，并确定了V结构域的核苷酸序列。互补决定区（CDR）的推导氨基酸序列表明，VH结构域的CDR3和VL结构域的CDR2可能与光损伤的识别有关。
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