Synthesis of cholesteryl supports and phosphoramidites containing a novel peptidyl linker for automated synthesis of triple-helix forming oligonucleotides (TFOs).
作者信息
Vu H, Singh P, Joyce N, Hogan M E, Jayaraman K
机构信息
Triplex Pharmaceutical Corporation, The Woodlands, TX 77380.
出版信息
Nucleic Acids Symp Ser. 1993(29):19-20.
PMID:8247764
Abstract
Preparation of G-rich TFOs containing cholesterol at the end presents challenging problems due to low yield in synthesis and loss in purification due to aggregation. These compounds also have low solubility which can result in variability in their uptake properties and cellular efficacy. To overcome these problems we have designed and synthesized cholesteryl -CPG (6) and -TentaGel (7) supports and phosphoramidite (8) containing a triglycyl linker.
摘要
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