人工水解核酸酶和核糖核酸酶的分子设计

Molecular design of artificial hydrolytic nucleases and ribonucleases.

作者信息

Komiyama M, Inokawa T, Shiiba T, Takeda N, Yoshinari K, Yashiro M

机构信息

Department of Industrial Chemistry, Faculty of Engineering, University of Tokyo, Japan.

出版信息

Nucleic Acids Symp Ser. 1993(29):197-8.

PMID:8247767

Abstract

Artificial nucleases and ribonucleases, which selectively hydrolyze the specific phosphodiester linkages in DNAs and RNAs, are prepared by the attachment of organic and inorganic catalysts to DNA oligomers as sequence-recognizing moieties. A hybrid of ethylenediamine and a 19-mer DNA selectively hydrolyzes tRNA(Phe) at the 3'-side of C63. Site-selective hydrolysis of DNAs has been accomplished by the lanthanide complexes bound to DNA oligomers. No concurrent oxidative cleavage of the ribose or the deoxyribose takes place.

摘要

人工核酸酶和核糖核酸酶可选择性水解DNA和RNA中的特定磷酸二酯键，它们是通过将有机和无机催化剂连接到作为序列识别部分的DNA寡聚物上制备而成的。乙二胺与19聚体DNA的杂交体可在C63的3'侧选择性水解tRNA（苯丙氨酸）。与DNA寡聚物结合的镧系元素配合物已实现了DNA的位点选择性水解。核糖或脱氧核糖没有同时发生氧化裂解。