Kasik J W, Rice E J
MetroHealth Medical Center, Case Western Reserve University, Cleveland, Ohio 44109.
Placenta. 1993 Jul-Aug;14(4):365-71. doi: 10.1016/s0143-4004(05)80458-8.
Two clones that are homologous to the mouse liver transferrin gene were isolated from a differential screen performed on a mouse cDNA library constructed from placenta. Using an insert derived from the larger of these clones as a template for the generation of random primed cDNA probes, northern blots were conducted against total RNA collected sequentially from placenta (7 days before birth to birth), maternal liver (7 days before birth to birth) and fetal liver (5 days before birth to birth). An approximately 2.3 kb message was detected in all three tissues which was upregulated in late gestation. Message was very abundant in both maternal and fetal liver, and present, but weak, in placenta. The clones were partially sequenced and both clones contain sequence that is identical to mouse liver transferrin. The data presented demonstrate an increase in mRNA transferrin in late gestation in maternal and fetal liver. Additionally, the placenta expresses a gene homologous to liver transferrin and it also is upregulated in late gestation.
从以胎盘构建的小鼠cDNA文库进行的差异筛选中，分离出两个与小鼠肝脏转铁蛋白基因同源的克隆。以这些克隆中较大的一个的插入片段为模板，生成随机引物cDNA探针，对依次从胎盘（出生前7天至出生）、母体肝脏（出生前7天至出生）和胎儿肝脏（出生前5天至出生）收集的总RNA进行Northern印迹分析。在所有三个组织中均检测到一条约2.3 kb的信使RNA，其在妊娠后期上调。信使RNA在母体和胎儿肝脏中都非常丰富，在胎盘中存在但较弱。对克隆进行了部分测序，两个克隆都包含与小鼠肝脏转铁蛋白相同的序列。所呈现的数据表明，母体和胎儿肝脏中mRNA转铁蛋白在妊娠后期增加。此外，胎盘表达与肝脏转铁蛋白同源的基因，并且在妊娠后期也上调。
