Dominguez S E, Menkel J L, Fairbrother A, Williams B A, Tanner R W
U.S. EPA Environmental Research Laboratory, Corvallis, Oregon 97333.
Toxicol Appl Pharmacol. 1993 Dec;123(2):226-33. doi: 10.1006/taap.1993.1241.
Bobwhite quail were exposed to 2,4-dinitrophenol (DNP) in a respirometer designed to continuously monitor exchange of O2 and CO2, from which metabolic rates (MR) were estimated. After 14-16 days of acclimation to the system (temperature 22 degrees C, light cycle 8L:14D), hens received feed amended with 0, 625, or 1250 ppm DNP ad libitum for 8 days, followed by 2-5 days of feed containing no DNP. The 625 ppm treatment produced dark-period MR 31-41% higher than corresponding control values, and light-period MR 23-32% higher than controls. The 1250 ppm treatment produced dark-period MR 48-77% higher than control values, and light-period MR 41-67% higher than controls. Over the 8 days of testing, the 625 ppm treatment hens expended 32% more energy than the control hens. Hens consuming 1250 ppm feed expended 60% more energy than control hens and lost most of their body fat. In general, the risk of being unable to meet energy demands for survival or reproduction would probably be substantially increased by the observed elevation in MR.
将白喉鹑置于一台用于持续监测氧气和二氧化碳交换的呼吸计中，使其接触2,4-二硝基苯酚（DNP），并据此估算其代谢率（MR）。在适应该系统14至16天（温度22摄氏度，光照周期为8小时光照：14小时黑暗）后，母鸡可随意采食添加了0、625或1250 ppm DNP的饲料，持续8天，随后2至5天采食不含DNP的饲料。625 ppm处理组的母鸡在黑暗期的代谢率比相应对照组高31%至41%，在光照期的代谢率比对照组高23%至32%。1250 ppm处理组的母鸡在黑暗期的代谢率比对照组高48%至77%，在光照期的代谢率比对照组高41%至67%。在8天的测试期内，625 ppm处理组的母鸡比对照组母鸡多消耗32%的能量。采食1250 ppm饲料的母鸡比对照组母鸡多消耗60%的能量，且大部分体脂流失。总体而言，观察到的代谢率升高可能会大幅增加无法满足生存或繁殖能量需求的风险。
