Teramoto K, Bowers J L, Kruskal J B, Clouse M E
Department of Radiology, New England Deaconess Hospital, Boston, Massachusetts.
Transplantation. 1993 Nov;56(5):1076-82. doi: 10.1097/00007890-199311000-00005.
The hepatic microcirculation in fatty and normal liver grafts in ACI rats was investigated using in vivo microscopy. Six groups were studied. They were: normal and fatty control livers (sham operated), 6-hr cold University of Wisconsin solution (UW)-preserved fatty and normal liver grafts (survival conditions, fatty and normal liver grafts), 18-hr cold UW-preserved fatty livers (nonsurvival conditions, fatty liver graft), and 24-hr cold UW-preserved normal livers (nonsurvival conditions, normal liver grafts). Fatty livers in all groups were found to have narrow and irregular sinusoids with blood cell adhesions to endothelial cells. The number of adhesions increased as the preservation time increased. Sinusoidal blood flow area decreased as the preservation time increased and was correlated with survival in both normal and fatty liver grafts. The phagocytic activity of Kupffer cells (corrected for flow) increased as the preservation time increased. The phagocytic Kupffer cell activity of the 18-hr preserved fatty liver group was greater than the activity of any other group. These features may cause liver cell death and contribute to primary graft nonfunction after transplantation of a fatty liver.
采用体内显微镜技术研究了 ACI 大鼠脂肪肝移植物和正常肝移植物的肝微循环。研究了六组。它们分别是：正常和脂肪肝对照组（假手术）、6 小时冷威斯康星大学溶液（UW）保存的脂肪肝移植物和正常肝移植物（存活条件下，脂肪肝移植物和正常肝移植物）、18 小时冷 UW 保存的脂肪肝（非存活条件下，脂肪肝移植物）以及 24 小时冷 UW 保存的正常肝（非存活条件下，正常肝移植物）。发现所有组中的脂肪肝都具有狭窄且不规则的肝血窦，血细胞与内皮细胞粘连。随着保存时间的增加，粘连数量增多。肝血窦血流面积随着保存时间的增加而减小，并且与正常肝和脂肪肝移植物的存活相关。库普弗细胞的吞噬活性（经血流校正）随着保存时间的增加而增强。保存 18 小时的脂肪肝组的库普弗细胞吞噬活性高于其他任何组。这些特征可能导致肝细胞死亡，并在脂肪肝移植后导致原发性移植物无功能。
